ABSTRACT
Tagetones A (1) and B (2), two new monocyclic diterpenoids were isolated from the n-hexane fraction of fresh flowers of Tagetes minuta L. (Asteraceae). Their structures were established by multiple spectroscopic methods (IR, HR-ESI-MS, and 1D-, and 2D-NMR), in addition to comparison with literature data. Compound 1 showed cytotoxic activity towards MCF7 and A549 cancer cells with IC values being 4.68 and 4.24 µmol·L, respectively, compared to doxorubicin (IC 0.13 and 1.12 µmol·L, respectively). Compound 2 also exhibited significant activity against HCT116 cancer cells (IC, 6.30 µmol·L).
Subject(s)
Humans , Cell Line, Tumor , Cell Survival , Diterpenes , Chemistry , Pharmacology , Flowers , Chemistry , Plant Extracts , Chemistry , Pharmacology , Tagetes , ChemistryABSTRACT
OBJECTIVE: To uncover the frequency and the spectrum of NPHS2 mutations in Egyptian children with non familial steroid-resistant nephrotic syndrome (SRNS). METHODS: Sixteen patients were screened by PCR-single-strand conformation polymorphism analysis of NPHS2 gene followed by direct sequencing. RESULTS: NPHS2 mutations were evident in four patients (25%) who were bearing four novel mutations including two frame shift mutations (R238fs and P45fs) and two missense mutations (I136L and F216Y). There were no phenotypic or histological characteristics of patients bearing NPHS2 mutations, apart from the earlier onset of the disease, compared to those who were not bearing mutations. CONCLUSION: NPHS2 mutations are prevalent in Egyptian children with non-familial SRNS and this may in part explain the less favorable prognosis reported in these patients.
Subject(s)
Child , Child, Preschool , Cross-Sectional Studies , Egypt/epidemiology , Frameshift Mutation , Genetic Markers , Humans , Infant , Intracellular Signaling Peptides and Proteins/blood , Membrane Proteins/blood , Mutation , Mutation, Missense , Nephrotic Syndrome/epidemiology , Polymerase Chain Reaction , Polymorphism, Single-Stranded Conformational , Prevalence , PrognosisABSTRACT
Poisoning of children is one of the most common and important subjects in the field of pediatric nursing. A poison is a substance which, when introduced into the body, injures or destroys the tissues and enzymes. The objective of this study was to determine the different types of poisoning occurring at Zagazig city to preschool children as well as to evaluate the immediate intervention offered by nurses to these cases. This study included 100 preschool age children attending at the Emergency Department at Zagazig University Hospital with poisoning. This study included also 15 nurses. The results of the present study showed that: 1-The majority of the studied children were five years or more. 2. The most common type of poisoning was due to corrosives. 3-Low socioeconomic standard and illiteracy of mothers were the leading cause of poisoning among preschool children. 4-There was a significant correlation between mothers' education and immediate care given at home. 5- The majority of nurses provided satisfactory nursing care. 6- Twenty percent only of nurses gave instructions about poisoning prevention. From the results of this study, the following can be recommended: 1- Continuous educational programs for nurses working at Emergency Department. 2- Poisoning prevention program to parents and children to prevent childhood poisoning which includes: Safe storage place for hazardous substances and medications, availability of ipecac at home avoidance of reused empty bottles in drinking, and the immediate care which should be given at home to different types of poisoning
Subject(s)
Humans , Male , Female , Child, Preschool , Nursing Care , Accidents, Home , Home Care Services , Health Education , Safety , Consumer Product Safety , Epidemiologic StudiesABSTRACT
The operation is only a brief phase in the continuing care of the pediatric surgical patients. To achieve success the proper assessment, diagnosing, pre and post operative nursing care is required by skillful nurses who are providing care for child and family. The current study focused on evaluating nursing intervention in pediatric surgical units in Cairo University Pediatric Hospital [CUPH]. A descriptive research design was adopted to conduct the current study. A convenience sample of 30 nurses was included. Tools for data collections were sociodemographic data sheet and observational checklist. The study's results revealed that most of the nurses nearly didn't provide dressing or prepare skin for operation and the majority of them had unsatisfactory level of performance. A significant relationship was detected between nurses' age and total score evaluation. It was recommended that an extensive training program on caring with children with surgical problems is a crucial need for development of nurses' performance in this vital unit